Research Internship Master 1 or 2: Deciphering the R-body Extension-Retraction Mechanism
Titre : Research Internship Master 1 or 2: Deciphering the R-body Extension-Retraction Mechanism Responsable : Schmatko Tatiana (MCUBE)
Description du projet R-bodies are unique molecular pistons produced by endosymbiotic bacteria that can switch in a fraction of second from self-enrolled 500nm ribbons to 20 microns membrane-perforating needles. Their extension is triggered by pH variation via a mechanism that remains unclear.
Within this internship the student will decipher R-bodies’ extension-retraction cycle dynamics
and mechanism by combining microfluidics, high speed imaging, force spectroscopy methods and theoretical models.The R-bodies are produced in E.coli and will be immobilized inside microfluidic chambers allowing rapid buffer exchange. We will assess the influence of buffer viscosity on R-bodies dynamics and the force generated during retraction using high speed imaging and optical tweezers. R-bodies will be trapped into giant unilamellar vesicles together with photoacid molecules and oscillatory rapid actuation will be triggered by light.
The longer term vision is to develop a biophysical model integrating the experimental
results and to ultimately tame this powerful and unique, nanomachine as a workhorse of future nanotechnology.